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                                                                                                                                                                                                                                                                                                                                                                                                    clinical chemistry & research solutions
                                                                                                                                                                                                                                                           3. Generation Total Antioxidant Status                            Total Oxidant Status




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                                      Research & Clinical Chemistry Assay Kits                                                                                                                                                                                        Reactive oxygen and nitrogen species are produced in metabolic   a sample is measured, and this is called total antioxidant capacity
                                                                                                                                                                                                                                                                      and  physiological  processes,  measurement  of  the  combined
                                                                                                                                                                                                                                                                                                                             (TAC),  total  antioxidant  activity  (TAA),  total  antioxidant  power


                                                                                                                                                                                                                                                                      nonenzymatic antioxidant capacity of biological fluids and other   (TAOP),  total  antioxidant  status  (TAS),  total  antioxidant
                                                                                                                                                                                                                                                                      samples  provides  an  indication  of  the  overall  capability  to   response(TAR), or other synonyms.

                                                                                                                                                                                                                                                                      counteract  reactive  oxygen  species  (ROS).  Under  certain



                                                                                                                                                                                                                                                                      conditions, the increase in oxidants and decrease in antioxidants   Serum (or plasma) concentrations of different oxidant species can
                          Total Antioxidant Status Assay Kit             Native Thiol Status Assay Kit                                                                                                                                                                cannot be prevented, and the oxidative stress or   in over 100   be measured in laboratories separately, but the measurements are

                                       TAS                                         -SH                   NEW                                                                                                                                                          disorders, develops                                    time-consuming,  labor-intensive  and  costly  and  require
                                                                                                                                                                                                                                                                                                                             complicated  techniques.  Since  the  measurement  of  different
                                                                                                                                                                                                                                                                      Antioxidant molecules prevent or inhibit these harmful reactions   oxidant molecules separately is not practical and their   oxidant

                            Total Oxidant Status Assay Kit                Dynamic Disulfide Status                                                                                                                                                                    Serum (or plasma) concentrations of different antioxidants can be   effects are additive, the total oxidant status (TOS) of a sample is
                                       TOS                                         -S-S                  NEW                                                                                                                                                          measured in laboratories separately, but the measurements are   measured and this is named total peroxide (TP), serum oxidation
                                                                                                                                                                                                                                                                      time-consuming, labor-intensive, costly, and require complicated   activity (SOA), reactive oxygen metabolites (ROM) or some other

                                                                                                                                                                                                                                                                      techniques. Because the measurement measurement of different   synonyms.
                               Oxidative Stress Index                    Total Thiol Status Assay Kit                                                                                                                                                                 antioxidant  molecules  separately  is  not  practical  and  their
                                       OSI                                         -SH                   NEW                                                                                                                                                          antioxidant effects are not additive, the total antioxidant capacity of                                                                                                                                     Princible of the Assay



                                                                                                                                                                                                                   • First and unique kit which reagents, calibrators and QC  Components                                                                                          Components                                                                                      Zinc found in the samples change the red-o









                                                                                                                                                                                                                     materials are liquid and stable.                                                                                                                                                                                                                             548 nm is proportional to total zinc level

                               Paraoxonase Assay Kit                           Zinc Assay Kit                                                                                                                                                                         R1 (Buffer)                       1 x  30 ml                                                                R1 (Buffer)                      1 x  30 ml
                                      PON1                                          Zn                   NEW                                                                                                       • Ready to use                                     R2 (ABTS Radical Cation)          1 x  8 ml                                                                 R2 (ABTS Radical Cation)         1 x  8 ml



                                                                                                                                                                                                                   • Colorimetric (660 nm)                            Standard                          1 x  4   ml          TOS Test Principle                                   Standard                         1 x  4   ml

                                Arylesterase Assay Kit                        Copper Assay Kit                                                                                                                     • Total Antioxidant Status Direct Measurement Kit  QC Level 1                        1 x  4   ml                                                               QC Level 1                       1 x  4   ml

                                       ARES                                         Cu                   NEW                                                                                                       • Fully automated and manually measurement options  QC Level 2                       1 x  4   ml          Oxidants  present  in  the  sample  oxidize  the  ferrous  ion–chelator   QC Level 2   1 x  4   ml
                                                                                                                                                                                                                                                                                                                             complex to ferric ion. The oxidation reaction is prolonged by enhancer

                                                                                                                                                                                                                   • Long lifetimes of the reagents, calibrators and QC materials                                            molecules, which are abundantly present in the reaction medium. The                                                                                        Sensitive and accurate



                                                                                                                                                                                                                                                                                                                             ferric ion makes a colored complex with chromogen in an acidic

                                                                                                                                                                                                                   • Suitable for serum, plasma, body fluids and tissue samples                                              medium.  The  color  intensity,  which  can  be  measured                                                                                                  Fast and convenient
                             Ischemia Modified Albumin                     Phosphorus (Inorganic)                                                                                                                                                                    TAS Test Principle                                      spectrophotometrically,  is  related  to  the  total  amount  of  oxidant   • First and unique kit which reagents and
                                       IMA                                          P                    NEW                                                                                                       • Suitable for plants, nutrients and oil extracts                                                         molecules  present  in  the  sample.  The  assay  is  calibrated  with     calibrators are liquid and stable.                                              High reagent stability and versatili

                                                                                                                                                                                                                   • High analytical performance charecteristics     Antioxidants in the sample reduce dark blue-green colored ABTS   hydrogen  peroxide  and  the  results  are  expressed  in  terms  of                                                                              Low interference in biological sampl
                                                                                                                                                                                                                                                                     radical to colorless reduced ABTS form. The change of absorbance   micromolar  hydrogen  peroxide  equivalent  per  liter  (µmol   • Ready to use
                                                                   Nadp-Dependent Isocitrate Dehyrogenase                                                                                                          • High precision                                  at 660 nm is related with total antioxidant level of the sample. The   H2O2Equiv./L)                                                                                                                               Easy to apply simple procedure
                                                                                NADP-IDH                 NEW                                                                                                       • High linearity                                  assay is calibrated with a stable antioxidant standard solution which                                          • Colorimetric (530 nm)

 ISO  ISO  ISO  OHSAS                                                                                                                                                                                                                                                is traditionally named as Trolox Equivalent that is a vitamin E analog .
 9001  14001  13485  18001                                                                                                                                                                                         • High sensitivity                                                                                                                                               • Total Oxidant Status Direct Measurement Kit                                                       Automated, colorimetric (548nm)
                                                                                                                                                                                                                                                                                                                                                                                    • Fully automated and manually measurement options                                                  Measuring in variety of samples


                                                                                Nitric Oxide                                                                                                                                                                                                                                                                                        • Long lifetimes of the reagents and calibrators

                                                                                   NO                     UD                                                                                                                                                                                                                                                                        • Suitable for serum, heparinized plasma, body

 C O N T A C T                                                                                                                                                                                                     Generation Comparison Table                                                               Oxidative Stress Index (OSI)
 Mücahitler Mahallesi 52012 Nolu Cadde                                                                                                                                                                                                                                  Rel Assay TAS                                                                                                 fluids and tissue samples

                                                                                                                                                                                                                                                                               ®
 No:10/1 Şehitkamil/Gaziantep/Türkiye                                                                                                                                                                                                        1. Generation  2. Generation  3. Generation                    TAS                TOS                                                  • High analytical performance charecteristics                                                   [Precision]
 Phone: +90 342. 230 37 52                                                                                                                                                                                          Reagents                 Lyophilized  Liquid        Liquid

 Fax: +90 342. 220 49 87                                                                                                                                                                                            Reagent Life             Short        Medium        Long (1 Year)                                                                                               • High precision                                                                                Intra-assay was determined on 30 replica
 e-mail: relassay@relassay.com                                                                                                                                                                                      Calibrators              Lyophilized  Lyophilized   Liquid                                                                                                      • High linearity                                                                                controls. The %CV results were 2.32 and

                                                                                                                                                                                                                    Calibrator Life          Short        Short         Long (1 Year)
                                                                                                                                                                                                                    QC Materials             Lyophilized  Lyophilized   Liquid                                                                                                      • High sensitivity                                                                              results were 3.54.
                                                                                                                                                                                                                    QC Material Life         Short        Short         Long (1 Year)                                                                                               • Available of lipoprotein  oxidation reaction curve

                                                                                                                                                                                                                    Tissue working           Interference  OK           OK                                           OSI

                                                                                                                                                                                                                    Linearity                Low          High          High
                                                                                                                                                                                                                                                                                                           OSI is a new indicator for oxidative stress.
                                                                                                                                                                                                                                                                                                    To determine  OSI, TAS and TOS levels measured and the results
                                                                                                                                                                                                                                                                                                               placed in the following table.
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